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Product Info.
FV1200 - Biological Confocal Laser Scanning Microscopes

The FLUOVIEW FV1200 biological confocal laser scanning microscope satisfies a range of researcher needs-enabling live cell imaging with improved high-sensitivity optical systems and delivering essential flexibility for diverse experimental arrangements.

 

Spectral and filter scan units provide two types of highprecision scanning.

Spectral Based Detection

  • Spectral detection using gratings for 2nm wavelength resolution and image acquisition matched to fluorescence wavelength peaks. User adjustable bandwidth of emission spectrum for acquiring bright images with minimal cross-talk.

Filter Based Detection

  • Three-channel scan unit with detection system featuring hard coated filter base. High-transmittance and high S/N ratio optical performance is achieved through integration of a pupil projection lens within the optics, the use of a high performance photomultiplier and an analog processing circuit with minimal noise.

New galvanometer Mirror

  • Our galvanometer mirror features an innovative silver coating that delivers outstanding reflective characteristics across a bandwidth from visible light to near infrared.

 

Ultra-high Sensitivity Detector with GaAsP Photomultiplier Tubes Further Enhances Quantum Efficiency
  • The ultra-high sensitivity detector makes it possible to view samples that were simply too dim to view with conventional equipment. The GaAsP PMT incorporates 2 channels and combines the images with a further 3 built-in channels as well as the channel transmitted from the detector.
    Maximum quantum efficiency is 45%, Peltier cooling holds noise down by 20%, and high S/N ratio imagescan be obtained under exceptionally low excitation light.

 

Dedicated Scanner for Photostimulation
  • Combination of the main scanner with a photostimulation scanner provide essential flexibility for tracking the diffusion or transport of fluorescence-labeled molecules or for marking specific live cells. The dual-fiber laser combiner makes it possible to use imaging lasers for photostimulation.

 

Silicone immersion objectives for live cell imaging deliver high-resolution
observation at depth
  • Silicone immersion objectives can be designed with a larger numerical aperture (NA) than water immersion objectives,
    increasing image resolution and brightness

UPLSAPO40×S

  • This new objective with intermediate magnification and high NA performance supports continuous focus with the IX3-ZDC.Continuous high-resolution observation during extended time-lapse imaging.(Scheduled to be available on 2013.)

    Magnification: 40x
    NA: 1.25 (silicone oil immersion)
    W.D.: 0.3mm
    Cover glass thickness: 0.15-0.19 mm
    Operation temperature: 23℃-37℃

    UPLSAPO40×S

UPLSAPO30×S

  • This low-magnification, high-NA objective delivers high-resolution imaging over a broad sample area. It enables continuous observation from low to high magnification when used with the zoom function of laser scanning microscopes.

    Magnification: 30x
    NA: 1.05 (silicone oil immersion)
    W.D.: 0.8mm
    Cover glass thickness: 0.13-0.19mm
    Operation temperature: 23℃-37℃

    UPLSAPO30×S

UPLSAPO60×S

  • This high-magnification, high-NA objective enables highly detailed imaging 

    NA: 1.30 (silicone oil immersion)
    W.D.: 0.3mm
    Cover glass thickness: 0.15-0.19mm
    Operation temperature:23℃-37℃

    UPLSAPO60×S

Enhance the reliability of colocalization analysis, with the low chromatic
aberration objective
  • This oil-immersion objective minimizes lateral and axial chromatic aberration in the 405-650nm spectrum, while supporting the reliable acquisition and measurement of colocalization images with superior positional accuracy. The objective also compensates for chromatic aberration through near infrared up to 850nm, making it an optimal choice for near infrared fluorescence observation

PLAPON60×OSC

  • Magnification: 60x
    NA: 1.4 (oil immersion)
    W.D.: 0.12mm
    Chromatic aberration compensation range:405-650nm

    PLAPON60×OSC

 

The IX3-ZDC Z Drift Compensator Offers a Range of Functionality for Autofocus

  • The IX3-ZDC uses low phototoxicity IR light to detect the correct focus position as set by the user. One-shot AF mode allows several focus positions to be set as desired for deeper samples, enabling efficient Z-stack acquisition in multi-position experiments. Continuous AF mode keeps the desired plane of observation precisely in focus, avoiding focus drift caused by temperature changes due to perfusion or reagent addition andmaking it ideal for measurements such as TIRF that requires more stringent focusing.

ZDC One-shot Function Detects Focus Fast, Even in High Magnification Observation

  • IX3-ZDC focus detection and tracking can be performed via the innovative touch panel independent of software. There's also a focus search function supported by a cell-safe, near-infrared laser enabling instant focusing on samples and start scanning.

 

Configurable Emission Wavelength

  • Select the dye name to set the optimal filters and laser lines

    Dye List

Re-Use Function

  • Open previously configured scanning conditions and apply them to new or subsequent experiments.

Wide Choice of Scanning Modes

  • Several available scanning modes including ROI, point and high-speed bidirectional scanning.

Dark Application Skin

  • Use of the dark application skin minimizes the influence of the noise from the screen for the sample.

 

 

  Spectral Version Filter Version
Laser light Violet/Visible Light Laser LD lasers: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635nm, 20mW
Multi-line Ar laser (458nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW)
AOTF Laser Combiner Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control
Continuously variable (0.1%–100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region
Fiber Broadband type (400nm–650nm)
Scanning and Detection Scanner module Standard 3 laser ports, Violet to IR
Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y)
Motorized optical port for fluorescence illumination and optional module adaptation, Adaptation to microscope fluorescence condenser
Detector module Standard 3 confocal Channels (3 photomultiplier detectors)
Additional optional output port light path available for optional units
6 position beamsplitter turrets with CH1 and CH2
CH1 and CH2 equipped with independent grating and slit for fast and flexible spectral detection
Selectable wavelength bandwidth: 1–100nm
Wavelength resolution: 2nm
Wavelength switching speed: 100nm/ms
CH3 with 6 position barrier filterturret
Standard 3 confocal Channels (3 photomultiplier detectors)
Additional optional output port light path available for optional units
6 position beamsplitter turrets with CH1 and CH2
CH1 to CH3 each with 6 position barrier filter turret (High performance filters)
Photo Detection Method 2 detection modes: Analog integration and hybrid photon counting
Scanning method 2 silver-coated galvanometer scanning mirrors
Scanning modes Scanning speed: 
512 x 512 (1.1 s, 1.6 s, 2.7 s, 3.3 s, 3.9 s, 5.9 s, 11.3 s, 27.4 s, 54.0 s)
bidirectional scanning 256 x 256 (0.064 s, 0.129 s), 512 x 512 (0.254 s)
X,Y,T,Z,λ
Line scanning: Straight line with free orientation, free line, Point scanning
X,Y,T,Z
Line scanning: Straight line with free orientation, free line, Point scanning
Pinhole Single motorized pinhole
pinhole diameter ø50–300μm (1μm step)
Single motorized pinhole
pinhole diameter ø50–800μm (1μm step)
Field Number (N.A.) 18
Optical Zoom 1x–50x in 0.1x increment
Z-drive Integrated motorized focus module of the microscope, minimum increment 0.01μm or 10nm
Transmitted light detector unit Module with integrated external transmitted light photomultiplier detector and 100W Halogen lamp, motorized switching, fiber adaptation to microscope frame
Microscope Motorized microscope Inverted IX83 (IX83P2ZF), Upright BX61, Upright focusing nosepiece & fixed stage BX61WI
Fluorescence illumination unit External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit
Motorized switching between LSM light path and fluorescence illumination
System Control Control Unit OS: Windows 7 Professional (English version), CPU: Intel Xeon E5-1620 (3.60GHz) or higher, Memory: 8GB (2GB x 4), Hard disk: 1 TB or more for data storage,
Dedicated I/F board: built-in control unit, Graphics board: NVIDIA Quadro 600, Optical drive: DVD ± R/RW Super-Multi
Power Supply Unit Galvo control boards, scanning mirrors and gratings, Real time controller Galvo control boards, scanning mirrors
Display SXGA 1280 x 1024, dual 19 inch (or larger) monitors or WQUXGA 2560 x 1600, 29.7 inch monitor
Optional unit SIM Scanner 2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port, Fiber introduction of near UV diode laser or visible light laser
Optional: 2nd AOTF laser combiner
TIRFM unit Available laser: 405–635 nm.
Motorized penetration ratio adjustment.
Automatic optical setting for TIRFM objectives
Ultra-high Sensitivity Detector Cooled GaAsP-PMT 2 channels
Fourth CH detector Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path
Fiber Port for Fluorescence Output port equipped with FC fiber connector (compatible fiber core 100–125μm)

Multi-dimensional Time-lapse Imaging with Outstanding Positional Accuracy

  • The FLUOVIEW FV1200 can be used for ideal multi dimensional time-lapse imaging during confocal observation, using multi-area time-lapse software to control the motorized XY stage and IX3-ZDC Z-drift compensator.

Significantly Improved Multi-Point Time-Lapse Throughput

  • Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system efficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single experiment to increase the efficiency of experiments. Microplates can be used to run parallel experiments, which significantly improves throughput for experiments that require long-termobservation.

    Supports repeated image acquisition from multiple areas in a single microplate well.

    Human lymphoblast cells TK6
    Courtesy of Masamitsu Honma, Dir.
    Biological Safety Research Center Div. of Genetics and Mutagenesis I, National Institute of Health Sciences

 

 

Combined Photostimulation and Imaging with Microsecond Precision Control

  • The SIM scanner system combines the main scanner with a photostimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous imaging of the effects of stimulation on the cell.

FLIP-Fluorescence Loss in Photobleaching

  • Fluorescence loss in photobleaching (FLIP) combines imaging with continuous bleaching of a specific region to observe the diffusion of a target protein within a cell. The changes in the image over time make it possible to observe the location of structural bodies that inhibit the diffusion of the molecule.

FRAP-Fluorescence Recovery after Photobleaching

  • Exposure of fluorescent-labeled target proteins to strong laser light causes their fluorescence to fade locally. Fluorescence recovery after photobleaching (FRAP) is used to observe the gradual recovery of fluorescence intensity caused by protein diffusion from the area surrounding the bleached region. By examining the resulting images, it is possible to characterize the diffusion speed of the molecule, and the speed of bindingand release between the molecule and cell structures.

Photoconversion

  • The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed to light of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes from green to red. This phenomenon can be used to mark individual Kaede-expressing target cells among a group of cells, by exposing them to laser light..

Uncaging

  • A 405nm laser is optional for uncaging with the SIM scanner system. Caged compoundscan be uncaged point-by-point or within a region of interest, while the main scanner of the FV1200 captures images of the response with no time delay.

Diffusion Measurement Package Extends Analytical Capabilities

  • This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular sizes and speeds.

RICS-Raster Image Correlation Spectroscopy

  • Raster image correlation spectroscopy (RICS) is a new method for analyzing the diffusion and binding dynamics of molecules in an entire, single image. RICS uses a spatial correlation algorithm to calculate diffusion coefficients and the number of molecules in specified regions. Cross correlation RICS (ccRICS) characterizes molecular interactions using fluorescent-labeled molecules in two colors.

FRAP Analysis

  • The Axelrod analytical algorithm is installed as a FRAP analysis method. The algorithm is used to calculate diffusion coefficients and the proportions of diffusing molecules.

High-level Magnification With High Resolution for the Broad-scope Imaging of
Large-scale Specimens

  • Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the images. The entire process from image acquisition to tiling can be fully automated.
    CNS markers in normal mice
    Objective : PLAPON60X
    Zoom : 2x
    Image acquisition numbers (XY): 32 x 38, 48 slices for each image
    Courtesy of Dr. Mark Ellisman PhD, Hiroyuki Hakozaki,
    MS Mark Ellisman
    National Center for Microscopy and Imaging Research (NCMIR),
    University of California, San Diego

Automated from 3D Image Acquisition to Mosaic Imaging

  • Multi-area time-lapse software automates the process from 3D image acquisition (using the motorized XY stage) to stitching. The software can be used to easily register wide areas, and the thumbnail display provides a view of the entire image acquired during the mosaic imaging process.

 

   fv1200_catalog.compressed.pdf (3.1M)
 
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